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Profacgen, a leading provider of life science research services, today announced the update of its Electrophoretic Mobility Shift Assay (EMSA) service, providing a powerful technique for studying protein-nucleic acid interactions.   Electrophoretic Mobility Shift Assay (EMSA), also known as gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common affinity electrophoresis technique used to study protein–DNA or protein–RNA interactions. This procedure can determine if a protein or mixture of proteins is capable of binding to a given DNA or RNA sequence, and can sometimes indicate if more than one protein molecule is involved in the binding complex.   EMSA can be used to study a wide range of protein–nucleic acid interactions, including: Transcription factor binding to DNA RNA binding proteins binding to RNA Protein-DNA interactions in gene regulation Protein-RNA interactions in microRNA processing Protein-DNA ... Continue reading →

Lactic acid bacteria (LAB) fermentation is a method of converting carbohydrates into lactic acid that employs LAB. Foods like yogurt, cheese, and sauerkraut are frequently fermented using a type of bacteria known as LAB. In addition to making biofuels and pharmaceuticals, LAB fermentation is also used to make food additives and preservatives.   How LAB Fermentation Works Anaerobic fermentation processes, such as LAB fermentation, function without the presence of oxygen. Lactic acid is created when LAB ferment carbohydrates, producing pyruvate first. The buildup of lactic acid lowers the food's pH, which prevents the growth of dangerous yeasts and bacteria.   Types of LAB Fermentation There are two main types of LAB fermentation: homolactic fermentation and heterolactic fermentation. Homolactic fermentation: In homolactic fermentation, LAB convert carbohydrates almost exclusively into lactic acid. This type of fermentation is used to produce ... Continue reading →

Profacgen, a leading provider of life science research services, today announced the update of its Electrophoretic Mobility Shift Assay (EMSA) service, providing a powerful technique for studying protein-nucleic acid interactions.   Electrophoretic Mobility Shift Assay (EMSA), also known as gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common affinity electrophoresis technique used to study protein–DNA or protein–RNA interactions. This procedure can determine if a protein or mixture of proteins is capable of binding to a given DNA or RNA sequence, and can sometimes indicate if more than one protein molecule is involved in the binding complex.   EMSA can be used to study a wide range of protein–nucleic acid interactions, including: Transcription factor binding to DNA RNA binding proteins binding to RNA Protein-DNA interactions in gene regulation Protein-RNA interactions in microRNA processing Protein-DNA ... Continue reading →

Within the cell, proteins continuously interact with each other to perform different functions. For some diseases where these functions are altered, blocking the binding between two or more proteins becomes a possible treatment.   Dr. Xavier Salvatella, a scientist led by ICREA researchers, published guidelines for the design of synthetic molecules to prevent interactions between the two proteins in Nature Communications. In short, researchers focus on the interaction of one protein with the alpha helix on the surface of another protein. This mechanism of interaction is very common and prevalent in cellular functions of therapeutic interest associated with diseases such as prostate cancer.   The guidelines presented in this work allow scientists to develop molecules in a relatively straightforward way that blocks any interaction between (potentially) globular proteins and α-helices, thus providing high versatility. These synthetic molecules also exhibit high stability, ... Continue reading →