Precise Pipetting Made Easy: Choosing the Right Pipette Tips

The precision and accuracy of even the best-calibrated pipette might be lost if the wrong type of tip is used. Depending on the experiment, the improper pipette tips can also cause contamination, waste of valuable samples or reagents, and even inflict bodily harm in the form of repetitive stress injury (RSI). There are numerous types of recommendations to pick from.There are a wide variety of tips to pick from. How do you determine which is ideal for your pipette and circumstance? Let us check this out in this blog:

Select high-quality pipette tips for accuracy and precision

Precision and accuracy are frequently the first factors that come to mind when deciding which tip type to use. Your pipetting will not be precise if there is any batch-to-batch, or even within-batch, variation in the form of the pipette tips. If the tip does not correctly fit your specific pipette, it may compromise the accuracy of your pipette. The drawn-in air can escape from your pipette barrel and tip if there is a weak seal, which prevents the proper amount of liquid from being inhaled. The ultimate volume supplied is therefore not entirely accurate. Choosing a tip that works well with your pipette might be challenging.

Pipette-specific or universal tips?

Using high-quality universal tips is the best option for your pipette and application. These universal tips are compatible with the vast majority of pipettes on the market. Universal tips have been developed to fit securely and firmly around all pipette tip cones, which vary somewhat in diameter from maker to manufacturer. Tips using the best technology, for example, are flexible at the proximal end of the tip (i.e., closest to the barrel), allowing them to suit a wider range of pipette types. In Microlit, you can find universal tips with all of the features discussed below (aerosol barrier, graduated, ergonomic, and so on).

Filter and non-filter Tips: Advantages and disadvantages

Filter tips and Non-filter tips are made for certain circumstances. If you're going to pipette something that could contaminate your pipette, such as volatile, corrosive, or viscous chemicals, you should think about Filter tips to safeguard your pipette and your samples.

Aerosol Barrier tips, also known as filter pipette tips, have a filter inside the proximal portion of the tip. The filter guards against aerosols and aspirating volatile or viscous liquids into the barrel, which can taint and harm the pipette. These tips are normally pre-sterilized and devoid of DNase/RNase. However, the term "barrier" is a bit misleading for some of these suggestions. Only a few high-end tips offer a true sealing barrier. Most filters just impede the flow of liquid into the pipette barrel. Because of the filter barrier in these tips, they are ideal for delicate applications such as qPCR.

The barrier eliminates PCR contamination by preventing sample spillover from the pipette, resulting in more reliable findings. Remember to perform your PCR positive and negative controls to detect sample carryover. Furthermore, filter tips are excellent 'training wheels' for newcomers. Pipette contamination occurs frequently when a new lab member mistakenly aspirates liquid into the pipette. Because liquid is in the piston, it is much easier and less expensive to discard a tip rather than send the entire pipette in for repair.

 Low retention tips

Low retention is an important feature regardless of whatever tip you use. Low-retention tips perform precisely what their name implies: they hold a small amount of liquid. If you've ever glanced at a typical pipette tip, you may have seen a small amount of liquid remaining after dispensing. Low-retention tips prevent this by containing a hydrophobic plastic additive that prevents liquid from adhering to the inside of the tips.

Ergonomic Tips

Pipetting, for example, can cause damaged joints and result in repetitive stress injury (RSI). As a result, firms have produced ergonomic tips that need reduced insertion and ejection forces, lowering the risk of RSI. Having said that, this feature is all about a good fit. An ergonomic tip is one that is specifically made to suit your pipette properly which reduces the risk of RSI.

Remember that you may typically obtain samples from various companies in order to determine what works best for your research.

To produce accurate and exact measurements, your pipette and tip must operate together. The number of suggestions available can be overwhelming. Using this guide will assist you in selecting the optimum tip for each application, resulting in cleaner, more robust results.

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