Innovative multichannel pipettes for handling liquids precisely

Multichannel pipettes have revolutionized the field of laboratory liquid handling by streamlining and expediting pipetting tasks. These versatile instruments are designed to simultaneously transfer multiple samples or reagents, significantly reducing the time and effort required for repetitive pipetting steps. With the ability to handle multiple channels, multichannel pipettes are ideal for scaling up experiments and increasing throughput without sacrificing accuracy and precision.

The most common multichannel pipettes are available in 8- or 12-channel configurations, perfectly spaced to work with 96- or even 384-well microplates. However, larger multichannel options and adjustable spacing versions cater to diverse experimental needs. By leveraging the capabilities of a multichannel pipette, researchers can accomplish in a few steps what would otherwise demand a much larger number of individual pipetting actions.

By employing a multichannel pipette in a scenario requiring 480 pipetting steps, one can accomplish the task with only 40 steps using a 12-channel pipette (5 rows of 8) or 60 steps with an 8-channel pipette (5 columns of 12). This expedited process not only accelerates plate filling but also minimizes the risk of repetitive strain injury (RSI) and the associated mental and physical fatigue. It is crucial to mitigate pipetting errors caused by fatigued analysts as they can compromise accuracy and precision, potentially leading to erroneous or inconclusive results. Consequently, repeating the assay becomes necessary, resulting in the wastage of valuable reagents, samples and reduced productivity.

While multichannel pipettes provide numerous advantages, they also present certain challenges, particularly concerning pipette tip attachment. Loading these tips can be cumbersome and often leads to uneven attachment. Difficulties such as problematic tip attachment, high ejection forces, and increased aspiration forces have impeded the proper utilization of multichannel pipettes. However, recent advancements in multichannel pipette technology have addressed these issues. For instance, electronic pipettes with electronic pistons and/or tip ejection mechanisms have been developed to overcome these challenges. Manufacturing breakthroughs have also improved tip attachment consistency, resulting in enhanced accuracy and precision. Nevertheless, it is important to note that even with these technological advancements, the critical factor for optimal performance lies in the proficiency of a well-trained operator who utilizes a calibrated multichannel pipette for each channel.

Enhancing Pipetting Proficiency: Essential Techniques for Multichannel Pipetting Success

To achieve accurate and reliable results when using multichannel pipettes, proper technique is of utmost importance, as operator variability can quickly lead to errors. Here are essential techniques to consider when utilizing these devices:

Mindful Tip Application: Always opt for manufacturer-approved pipette tips and select those with the smallest air gap. After aspirating the sample, compare the liquid levels within the tips. If uneven, it may indicate inconsistent tip placement across the pipette channels.

Pre-Wet Pipette Tips: Pre-wetting the pipette tips is crucial. This process humidifies the captive air space (air cushion) within the tips, minimizing sample evaporation and the risk of pipette dripping.

Slow and Steady Aspiration: Rapid plunger movements or fast aspiration can introduce unwanted bubbles within the pipette tips. To avoid this, ensure a controlled and gradual aspiration process.

Vertical Aspiration, Minimizing Angles: When aspirating, it is important to maintain a vertical position without tilting or angling the pipette. Angling the pipette in various directions, such as forward-backward or left-right, can result in uneven immersion depths of the pipette tips, leading to inconsistent volume transfer.

Essential Pause After Aspiration: Once the plunger is released, pause for a full second with the tips still immersed in the sample. This pause allows the total sample volume to fully enter each pipette tip, ensuring uniformity across all channels.

Adjusting Dispense Speed: Sometimes, a slower dispense speed may result in residual samples remaining in the pipette tips. If you observe residual droplets, it may indicate the need to adjust the dispense speed to a faster rate. This adjustment ensures that the captive air space above the sample generates enough force to push the entire sample out of the pipette tips.

Tip-Touch or Brush-Off Technique: During dispensing, whenever feasible, gently touch the pipette tips against the side walls of the plate or receptacle. Alternatively, you can dispense the sample on top of the solution. These actions create added surface tension, aiding in complete sample removal and reducing the likelihood of residual sample retention.

By employing these techniques, you can enhance the efficiency and accuracy of multichannel pipetting, ensuring complete sample transfer and minimizing potential sources of error.

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