MicroRNA Stability in FFPE Tissue Samples

What are miRNAs?

miRNA stands for micro ribonucleic acid. It refers to the short non-coding RNAs regulating gene expression at the post transcription stage. It usually binds to the untranslated region of the target mRNA, repressing protein production. However, the exact mechanism of miRNA-mediated protein repression is still unknown. Modifications of miRNAs mediated gene expression significantly affect the human health status and cause severe disease conditions. Therefore, studies on archived patient tissue samples are propelling to understand the exact miRNA role functions.

Stability of microRNA in FFPE Tissues

FFPE samples are one form of preserving valuable tissue samples from patients and donors for research. It is mainly done to store the tissue samples for longer years by preventing tissue apoptosis and necrosis, maintaining sample integrity. Recently, microRNA stability in FFPE tissues has gained more interest and given hope to molecular researchers. It helps the researchers to extend various microarray and DNA sequencing studies on human tissues. Also, in comparison to the long RNAs, microRNAs are found to be more stable at extreme temperatures and pH and, therefore, can withstand the formalin fixation process to produce FFPE samples. However, the nucleic acid degradation is more in the FFPE samples than frozen tissue samples with no significant changes in the histological characteristics. Therefore, FFPE samples allow deep genetic sequencing and data processing offering more powerful insights into disease pathogenesis.

Clinically Graded FFPE Samples Available Online

Human tissues research has evolved over the years. Well-preserved, high-quality tissue samples in the format of frozen and FFPE samples accelerate the studies on human diseases. Nowadays, biomedical researchers strive to obtain an understanding of disease pathogenesis and human biology on a genetic level. Human tissues extracted from patients provide substantial evidence for the drug and diagnostic discovery. As the number of incidences is increasing, there is a pressing demand for clinically graded, well-preserved human tissue samples and FFPE samples. Bringing an end to the long-term search of ideal human tissue samples, Central BioHub provides access to thousands of pathologist-certified frozen tissue samples or FFPE blocks online. Dive into their latest inventory here and order samples today: https://centralbiohub.de/Biospecimens/Cancer-Samples/FFPE-Samples. Central BioHub is a first-ever digital marketplace bringing millions of human biospecimens for research. It offers highly characterized samples tested for specific clinical parameters to add validity to your research. Visit www.centralbiohub.de for more details on the inventory and procurement process.

Article source: https://article-realm.com/article/Sports-Recreation/31342-MicroRNA-Stability-in-FFPE-Tissue-Samples.html